A glimpse on new biomarker imaging from INNOVIDEA & FLI
Living tissue have capability to absorb and scatter photon at different depth, depending on light wavelength. For long, Near Infrared (NIR-I i.e. 700 to 950nm) portion of spectrum have demonstrated good penetration capability and have allowed various fluorescence imaging for in-vivo, thanks to contrast agent or dyes called fluophore. Several imaging techniques in this band were possible thanks to highly sensitive CDD or S-CMOS for the benefit of biomedical research or clinical application.
Recently, the shift to higher wavelength fluorescence imaging in SWIR or NIR II (1000nm to 1350nm) region has drawn great attention worldwide. Here are a few example of research and innovation in this field:
New fluorescent nanomaterials called NIR-I to NIR-II – such as carbon nanotubes, quantum dots or polymer nanoparticles – replace or complement fluophore. They allow imaging in SWIR band, their light emission when excited in the NIR band. This reduces interference of photon absorption, scattering and autofluorescence.
Recent progress in fluorescent DNA aptamers permits to recognize cancer biomarkers on soluble or membrane bound form. Other works conducted this time with RNA aptamer tags have open the possibility to look into intracellular processes for better understanding cellular signaling and physiology.
Another promising technology for instance makes use of SWIR light for exciting various rare earth nanocrystals dyes that accumulate at disease sites. It allows earlier detection of cancer than MRI or NIR imaging.Finally, for medical use, existing probes like the Indocyanine Green (ICG) which is FDA approved and widely used, is now known to have strong emission in the infrared, greatly simplifying the process for new therapeutic applications or experimentations.
The scope of applications is large, especially in cancer domain but not only, as can be seen in this non exhaustive list:
- In-vivo pre and post treatment,
- Therapy, or sometimes both diagnosis and therapy at same time,
- Surgery (suppressing need for lymph node biopsies),
- Cardiovascular lesion,
- Mass spectrometry imaging, …
The C-RED 2 cameras from First Light Imaging use at -40°C have shown their outstanding capability thanks to the unique combination of performances (readout noise <30 electrons, 600FPS frame rate and 93dB dynamic range at the same time). They outperform deeper cooled cameras at a more affordable cost.
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